PROJECT TITLE: International Collaborative Study for the Validation of a HILIC-MS/MS Method for Analysis of Paralytic Shellfish Toxins and Tetrodotoxin in Live Bivalve Molluscs
Time frame: January 2017 to September 2018
Principal Investigator: Dr Andrew D. Turner
Address: Centre for Environment Fisheries and Aquaculture Science (Cefas)
Tel/Fax: +44 (0)1305 206636 / +44 (0)1305 206601
Home page URL: https://www.cefas.co.uk/
Other key persons: Dr Tim Harwood, Cawthron Institute, New Zealand
Objectives: The aim of this project will be to conduct a Collaborative Study of the LC-MS/MS method for PSP toxins in shellfish, using a suitable number of international toxin testing laboratories. The work will require active collaboration with organisations worldwide to validate the method, ultimately demonstrating its fitness for purpose for official control testing. The final achievement will involve the delivery of an extensive data set, the publication of the collaborative study and ultimately the delivery of a formally-approved official method of analysis.
The project will be conducted through a collaborative study lead by Dr Andrew Turner at Cefas together in partnership with Dr Tim Harwood at Cawthron Institute. Additional guidance and collaboration will be provided by the EURLMB. The project has been discussed with international collaborators and is supported by Prof Ana Gago-Martinez Director of the EURLMB and James Hungerford, USFDA and Co-chair of AOAC Marine Toxin Task Force.
Implementation progress (November 6th, 2019): The PST by LC-MS/MS method ("Boundy Method") developed by Cawthron in collaboration with CEFAS in the UK has been published 25th October 2019. This method can supersede the Lawrence method as the confirmatory method for PST monitoring in New Zealand. We will continue to apply the Boundy method on all samples we receive for PST testing.
Andrew D. Turner, Monika Dhanji-Rapkova, Sum Y. T. Fong, James Hungerford, Michael J. Boundyand, D. Tim Harwood. (2019) Ultrahigh-Performance Hydrophilic Interaction Liquid Chromatography with Tandem Mass Spectrometry Method for the Determination of Paralytic Shellfish Toxins and Tetrodotoxin in Mussels, Oysters, Clams, Cockles, and Scallops: Collaborative Study. Journal of AOA C International Vol. 103, in press.